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Optical Fractionator Workflow

Each step in the workflow includes context-sensitive help () to assist you.

1. Click Probes>Optical Fractionator Workflow to open the Optical Fractionator Workflow window.
2. You are prompted to start a new subject (Start a new subject) or to continue with a subject (Load subject data from existing file).

If you select Load subject data from existing file, Microlucida typically directs you to the Count Objects step. Because the workflow is streamlined, only the relevant steps are displayed and they are re-numbered accordingly (i.e., Step 10 in the complete workflow might be referred to as Step 4 in the streamlined workflow).

3. Follow the steps in the workflow.

Workflow

Indicate the Areas Used for Counting

• Step 1: Set up the Subject

• Step 2: Set Microscope to Low Magnification
• Step 3: Trace your Region(s) of Interest
• Step 4: Set Microscope to High Magnification

Define Probe Configuration

• Step 5: Measure Mounted Thickness
• Step 6: Define the Counting Frame Size
• Step 7: Define SRS Grid Layout
• Step 8: Define Disector Options
• Step 9: Save Sampling Parameters

Perform Counting

• Step 10: Count Objects
• Step 11: View the Sampling Results

Watch these webinars: Unbiased Stereology to determine the number of cells in a region of interest, Implementing the Optical Fractionator Workflow

See Optical Fractionator for more information.

Microlucida 3 | MBF Bioscience Support Center | Downloads